Home > Aberrant activation of cell cycle-related kinases in uterine leiomyosarcoma
Aberrant activation of cell cycle-related kinases in uterine leiomyosarcomathe potential therapeutic impact of PLK1 or CHEK1 inhibition
March 18, 2022
Nagoya University
National Cancer Center Japan
Key Points
- The authors identified novel therapeutic drug candidates for uterine leiomyosarcoma.
- The authors revealed the unique gene expression profile of uterine leiomyosarcoma through transcriptome analysis.
Summary
Uterine leiomyosarcoma (ULMS) is one of the most aggressive gynecological malignancies and no effective treatment strategies have been established. This study aimed to identify novel therapeutic targets for ULMS based on transcriptome analysis and assess the preclinical efficacy of novel drug candidates. Transcriptome analysis was performed using fresh-frozen samples of six ULMS and three myoma samples. The authors identified 512 genes that were significantly dysregulated in uterine leiomyosarcoma compared with myoma. Ingenuity Pathway Analysis revealed that the function of several genes, including CDK1, CDK2, AURKB, CHEK1, and PLK1, were predicted to be activated in ULMS. Then, the inhibitory effects of several selective inhibitors for the candidate genes were investigated using ULMS-derived cell lines. Of them, PLK1 or CHEK1 inhibitors (BI-2536 or prexasertib) were found to exert a superior anti-cancer effect against cell lines at low nanomolar concentrations and induce cell cycle arrest. Moreover, cytotoxic drugs synergistically enhanced the effect of prexasertib. In the mice model, BI-2536 monotherapy remarkably suppressed tumorigenicity. Moreover, the prexasertib and cisplatin combination therapy inhibited tumor proliferation and prolonged the time to tumor progression. In conclusion, cell cycle-related kinases may present a promising therapeutic strategy for the treatment of ULMS.
Research Background
Uterine leiomyosarcoma (ULMS) is among the most aggressive gynecological malignancies. To achieve complete resection, total hysterectomy offers the best chance of cure for localized ULMS. However, the risk of recurrence after complete resection reaches within the range of 50%–70%. For recurrent and metastatic ULMS, no effective treatment strategies have been established. Therefore, the median overall survival of patients with metastatic ULMS is found to be one or two years. Recently, novel agents, such as trabectedin, pazopanib, and eribulin, were approved for soft-tissue sarcomas. Despite the high therapeutic expectations regarding these agents, the prognosis of patients with ULMS did not considerably improve. Therefore, the clinical outcome of ULMS remains unsatisfactory, and new therapeutic agents are urgently needed.
Research Results
Transcriptome analysis was performed using six ULMS and three myoma samples. There were 387 and 125 significantly upregulated and downregulated genes, respectively, in ULMS compared with myoma. To assess the putative function of the 512 DEGs, pathway analysis was performed using the IPA software, which revealed that several pathways associated with cell cycle and DNA damage checkpoint were significantly dysregulated. For instance, these pathways included the Kinetochore Metaphase Signaling Pathway (p = 5.01E-24), Mitotic Roles of Polo-Like Kinase (p = 1.58E-11), and Cell Cycle: G2/M DNA Damage Checkpoint Regulation (p = 2.51E-7). In addition, from the upstream regulator analysis using IPA, it was observed that the function of CDK1, AURKB, PLK1, CHEK2, CHEK1, CDK2, and PRKDC was significantly activated in ULMS. The results were validated using three GEO datasets. Therefore, the authors considered the activated kinases as potential therapeutic targets for ULMS. Through an in vitro drug screening, PLK1 or CHEK1 (BI-2536 or prexasertib) inhibitors were found to exert a superior anti-cancer effect against cell lines at low nanomolar concentrations and induce cell cycle arrest. Moreover, in mice model, BI-2536 monotherapy remarkably suppressed tumorigenicity, and the prexasertib and cisplatin combination therapy inhibited tumor proliferation.
Research Summary and Future Perspective
The authors identified upregulated expressions of PLK1 and CHEK1; their kinase activity was activated in uterine leiomyosarcoma. BI-2536 or prexasertib inhibitors demonstrated a significant anti-cancer effect. Moreover, according to clinical trials in other malignancies, the toxicity of these inhibitors was well tolerated. Therefore, PLK1 or CHEK1 inhibition is a promising therapeutic strategy that might improve clinical outcomes in patients with ULMS.
Publication
Journal
Clinical Cancer Research
Title
Aberrant activation of cell cycle-related kinases and the potential therapeutic impact of PLK1 or CHEK1 inhibition in uterine leiomyosarcoma
Authors
Kosuke Yoshida1,2,3, Akira Yokoi1,2, Tomofumi Yamamoto3, Yusuke Hayashi3, Jun Nakayama3, Tsuyoshi Yokoi4, Hiroshi Yoshida5, Tomoyasu Kato6, Hiroaki Kajiyama1, Yusuke Yamamoto3
1 Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya, Japan
2 Institute for Advanced Research, Nagoya University, Nagoya, Japan
3 Laboratory of Integrative Oncology, National Cancer Center Research Institute, Tokyo, Japan
4 Department of Drug Safety Sciences, Division of Clinical Pharmacology, Nagoya University Graduate School of Medicine, Nagoya, Japan
5 Department of Diagnostic Pathology, National Cancer Center Hospital, Tokyo, Japan
6 Department of Gynecology, National Cancer Center Hospital, Tokyo, Japan
DOI
10.1158/1078-0432.CCR-22-0100
Contact
- Hiroaki Kajiyama
Professor
Department of Obstetrics and Gynecology
Nagoya University Graduate School of Medicine - Akira Yokoi
Assistant Professor
Department of Obstetrics and Gynecology
Nagoya University Graduate School of Medicine - Kosuke Yoshida
Project Assistant Professor
Department of Obstetrics and Gynecology
Nagoya University Graduate School of Medicine - Tomoyasu Kato
Chief
Department of Gynecology
National Cancer Center Hospital - Yusuke Yamamoto
Unit Leader
Laboratory of Integrative Oncology
National Cancer Center Research Institute
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