Annual Report 2019
Department of Analytical Pathology
Nobuyoshi Hiraoka, Yoshinori Ino-Ishikawa, Atsuko Nakazawa
Introduction
In the Department of Analytical Pathology, the pathobiological and clinicopathological characteristics of target molecules are analyzed to evaluate their potential significance in applying diagnostic or treatment use in future. Expression of the molecules or genes in human tissues is assessed by morphological techniques, immunohistochemistry, RT-PCR, in situ hybridization, etc., and the results are compared to clinicopathological information. We also try to develop new, more reliable, or more effective analytical methods and tools.
Research activities
The tumor immune microenvironment in pancreatic cancer is studied clinicopathologically and pathobiologically. We found that endothelial cells in the cancer tissue have dysfunctional expressions of adhesion molecules resulting in a decrease in tumor-infiltrating lymphocytes. We obtained a new compound that accelerates tumor-infiltrating lymphocytes and the antitumor immune response by recovering VCAM-1 expression on the endothelial cells in cancer tissue.
Education
The analytical techniques were inculcated to technicians and researchers in several departments of the National Cancer Center.
Future prospects
We will answer requests regarding the selected project in various types of study containing basic, preclinical, and clinical studies and assess the clinicopathological or pathobiological significance of the target molecules. We will develop methods of quantitative analysis to evaluate morphological findings that currently analyzed qualitatively.
List of papers published in 2019
Journal
1. Yonemaru J, Takahashi M, Nara S, Ichikawa H, Ishigamori R, Imai T, Hiraoka N. A yolk sac tumor of the pancreas and derived xenograft model effectively responded to VIP chemotherapy. Pancreatology, 20:551-557, 2020
2. ICGC/TCGA Pan-Cancer Analysis of Whole Genomes Consortium. Pan-cancer analysis of whole genomes. Nature, 578:82-93, 2020
3. Ikeda K, Sato S, Chigira H, Shibuki Y, Hiraoka N. Characterizing the Effect of Automated Cell Sorting Solutions on Cytomorphological Changes. Acta Cytol, 64:232-240, 2020
4. Ikeda K, Sato S, Chigira H, Shibuki Y, Hiraoka N. Effects of the Storage Solution Type and Prolonged Storage on the Immunoreactivity of Cells. Acta Cytol, 64:352-359, 2020
5. Ishida T, Nara S, Akahoshi K, Takamoto T, Kishi Y, Esaki M, Hiraoka N, Shimada K. Left hepatic trisectionectomy for perihilar cholangiocarcinoma with a right-sided round ligament: A case report. World J Gastrointest Surg, 12:68-76, 2020
6. Sunami K, Ichikawa H, Kubo T, Kato M, Fujiwara Y, Shimomura A, Koyama T, Kakishima H, Kitami M, Matsushita H, Furukawa E, Narushima D, Nagai M, Taniguchi H, Motoi N, Sekine S, Maeshima A, Mori T, Watanabe R, Yoshida M, Yoshida A, Yoshida H, Satomi K, Sukeda A, Hashimoto T, Shimizu T, Iwasa S, Yonemori K, Kato K, Morizane C, Ogawa C, Tanabe N, Sugano K, Hiraoka N, Tamura K, Yoshida T, Fujiwara Y, Ochiai A, Yamamoto N, Kohno T. Feasibility and utility of a panel testing for 114 cancer-associated genes in a clinical setting: A hospital-based study. Cancer Sci, 110:1480-1490, 2019
7. Sone M, Arai Y, Sugawara S, Kubo T, Itou C, Hasegawa T, Umakoshi N, Yamamoto N, Sunami K, Hiraoka N, Kubo T. Feasibility of genomic profiling with next-generation sequencing using specimens obtained by image-guided percutaneous needle biopsy. Ups J Med Sci, 124:119-124, 2019
8. Hiraoka N, Toue S, Okamoto C, Kikuchi S, Ino Y, Yamazaki-Itoh R, Esaki M, Nara S, Kishi Y, Imaizumi A, Ono N, Shimada K. Tissue amino acid profiles are characteristic of tumor type, malignant phenotype, and tumor progression in pancreatic tumors. Sci Rep, 9:9816, 2019
9. Hironaka-Mitsuhashi A, Tsuda H, Yoshida M, Shimizu C, Asaga S, Hojo T, Tamura K, Kinoshita T, Ushijima T, Hiraoka N, Fujiwara Y. Invasive breast cancers in adolescent and young adult women show more aggressive immunohistochemical and clinical features than those in women aged 40-44 years. Breast Cancer, 26:386-396, 2019
10. Iwasaki T, Hiraoka N, Ino Y, Nakajima K, Kishi Y, Nara S, Esaki M, Shimada K, Katai H. Reduction of intrapancreatic neural density in cancer tissue predicts poorer outcome in pancreatic ductal carcinoma. Cancer Sci, 110:1491-1502, 2019
11. Kobayashi K, Yoshimoto S, Matsumoto F, Ando M, Murakami N, Omura G, Fukasawa M, Matsumoto Y, Matsumura S, Akamatsu M, Hiraoka N, Eigitsu R, Mori T. All-Exon TP53 Sequencing and Protein Phenotype Analysis Accurately Predict Clinical Outcome after Surgical Treatment of Head and Neck Squamous Cell Carcinoma. Ann Surg Oncol, 26:2294-2303, 2019
12. Narumi K, Miyakawa R, Shibasaki C, Henmi M, Mizoguchi Y, Ueda R, Hashimoto H, Hiraoka N, Yoshida T, Aoki K. Local Administration of GITR Agonistic Antibody Induces a Stronger Antitumor Immunity than Systemic Delivery. Sci Rep, 9:5562, 2019
13. Saito Y, Muramatsu T, Kanai Y, Ojima H, Sukeda A, Hiraoka N, Arai E, Sugiyama Y, Matsuzaki J, Uchida R, Yoshikawa N, Furukawa R, Saito H. Establishment of Patient-Derived Organoids and Drug Screening for Biliary Tract Carcinoma. Cell Rep, 27:1265-1276.e4, 2019
14. Shiino S, Yoshida M, Jimbo K, Asaga S, Takayama S, Maeshima A, Tsuda H, Kinoshita T, Hiraoka N. Two rare cases of endosalpingiosis in the axillary sentinel lymph nodes: evaluation of immunohistochemical staining and one-step nucleic acid amplification (OSNA) assay in patients with breast cancer. Virchows Arch, 474:633-638, 2019
15. Hatano M, Ojima H, Masugi Y, Tsujikawa H, Hiraoka N, Kanai Y, Shimada K, Shinoda M, Sakamoto M. Steatotic and nonsteatotic scirrhous hepatocellular carcinomas reveal distinct clinicopathological features. Hum Pathol, 86:222-232, 2019
16. Asano D, Nara S, Kishi Y, Esaki M, Hiraoka N, Tanabe M, Shimada K. A Single-Institution Validation Study of Lymph Node Staging By the AJCC 8th Edition for Patients with Pancreatic Head Cancer: A Proposal to Subdivide the N2 Category. Ann Surg Oncol, 26:2112-2120, 2019
Book
1. Singhi AD, Adsay NV, Hiraoka N, Terris B. Acinar cystic transformation of the pancreas. In: WHO classification of Tumours (ed), Digestive System Tumours WHO Classification of Tumours, 5th Edition, Lyon, IARC, pp 300-302, 2019
2. Singhi AD, Adsay NV, Hiraoka N, Terris B. Serous neoplasms of the pancreas. In: WHO classification of Tumours (ed), Digestive System Tumours WHO Classification of Tumours, 5th Edition, Lyon, IARC, pp 303-306, 2019